Cover: A Preliminary Model of Double-Minute-Mediated Gene Amplification

A Preliminary Model of Double-Minute-Mediated Gene Amplification

Published 1983

by Paul F. Morrison, Jerry Aroesty, S. P. Creekmore, P. E. Barker, Thomas L. Lincoln

Purchase Print Copy

 Format Price
Add to Cart Paperback45 pages $23.00

Develops a mathematical model of double minute (dm) population dynamics based on current concepts of the saltatory replication, random partitioning, nuclear exclusion and loss, and cellular growth inhibition of these extrachromosomal elements. A highly accurate approximate analytical solution has been obtained for the dm frequency distribution at steady state, and preliminary analysis of transient states has been performed. The steady-state solution has been fit to experimental frequency data of the SW527N carcinoma line, the excellent goodness of fit (chi squared = 2.6, d.f. = 29) providing preliminary evidence of the consistency of this set of mechanisms. Two special cases are examined. The model predicts that the population-average rate of extrareplicative dm production is .039 +/- S.E.009 dms/hr/cell in the first case and is tenfold higher than when such replication occurs on the chromosome alone. It is found that dm-related growth inhibition can be nearly as high as that observed for the S180 sarcoma lines (about 0.5 percent per dm lengthening of the doubling time) or as low as zero.

This report is part of the RAND note series. The note was a product of RAND from 1979 to 1993 that reported other outputs of sponsored research for general distribution.

This document and trademark(s) contained herein are protected by law. This representation of RAND intellectual property is provided for noncommercial use only. Unauthorized posting of this publication online is prohibited; linking directly to this product page is encouraged. Permission is required from RAND to reproduce, or reuse in another form, any of its research documents for commercial purposes. For information on reprint and reuse permissions, please visit

RAND is a nonprofit institution that helps improve policy and decisionmaking through research and analysis. RAND's publications do not necessarily reflect the opinions of its research clients and sponsors.